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Étude suédoise revue par les pairs : oui l’ARN de la protéine spike est rétro-transcrit en ADN (définitivement) dans des cellules de foie in vitro. Pire, ce dernier semble ACTIVER les rétrotranscriptases humaines. « Impossible ! » avaient hurlé les idiots. https://t.co/lg8lr0U8Cj
— Idriss J. Aberkane Ph.D, Ph.D & Ph.D (@idrissaberkane) August 9, 2022
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SOURCE, 1, 1, 1, 1, 2 and 1,*
1Department of Clinical Sciences, Lund University, 20502 Malmö, Sweden2Infection Medicine, Department of Clinical Sciences, Lund University, 22362 Lund, Sweden*Author to whom correspondence should be addressed.Academic Editor: Stephen MalnickCurr. Issues Mol. Biol. 2022, 44(3), 1115-1126; https://doi.org/10.3390/cimb44030073Received: 18 January 2022 / Revised: 19 February 2022 / Accepted: 23 February 2022 / Published: 25 February 2022(This article belongs to the Topic Clinical, Translational and Basic Research on Liver Diseases)Abstract
Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.Keywords: COVID-19 mRNA vaccine; BNT162b2; liver; reverse transcription; LINE-1; Huh7
